2. Sample size Explain how the sample size was decided. Provide details of any a priori sample size calculation, if done. examples
Example 1
“The sample size calculation was based on postoperative pain numerical rating scale (NRS) scores after administration of buprenorphine (NRS AUC mean = 2.70; noninferiority limit = 0.54; standard deviation = 0.66) as the reference treatment…and also Glasgow Composite Pain Scale (GCPS) scores…using online software (Experimental design assistant; https://eda.nc3rs.org.uk/eda/login/auth). The power of the experiment was set to 80%. A total of 20 dogs per group were considered necessary.” [1]
Example 2
“We selected a small sample size because the bioglass prototype was evaluated in vivo for the first time in the present study, and therefore, the initial intention was to gather basic evidence regarding the use of this biomaterial in more complex experimental designs.” [2]
- Bustamante R, Daza MA, Canfrán S, García P, Suárez M, Trobo I and Gómez de Segura IA (2018). Comparison of the postoperative analgesic effects of cimicoxib, buprenorphine and their combination in healthy dogs undergoing ovariohysterectomy. Veterinary Anaesthesia and Analgesia. doi: 10.1016/j.vaa.2018.01.003
- Spin JR, Oliveira GJPLd, Spin-Neto R, Pires JR, Tavares HS, Ykeda F and Marcantonio RAC (2015). Avaliação histomorfométrica da associação entre biovidro e osso bovino liofilizado no tratamento de defeitos ósseos críticos criados em calvárias de ratos. Estudo piloto. Revista de Odontologia da UNESP. http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-25772015000100037&nrm=iso