Recommended set

16. Animal care and monitoring Describe the humane endpoints established for the study, the signs that were monitored, and the frequency of monitoring. If the study did not have humane endpoints, state this. examples

16a Describe any interventions or steps taken in the experimental protocols to reduce pain, suffering, and distress.
16b Report any expected or unexpected adverse events.
16c Describe the humane endpoints established for the study, the signs that were monitored, and the frequency of monitoring. If the study did not have humane endpoints, state this.
Explanation
Examples

Example 1

“Both the research team and the veterinary staff monitored animals twice daily. Health was monitored by weight (twice weekly), food and water intake, and general assessment of animal activity, panting, and fur condition…The maximum size the tumors allowed to grow in the mice before euthanasia was 2000 mm3.” [1]

Muscella A, Vetrugno C, Cossa LG, Antonaci G, De Nuccio F, De Pascali SA, Fanizzi FP and Marsigliante S (2016). In Vitro and In Vivo Antitumor Activity of [Pt(O,O'-acac)(gamma-acac)(DMS)] in Malignant Pleural Mesothelioma. PLoS One. doi: 10.1371/journal.pone.0165154