13. Objectives Clearly describe the research question, research objectives and, where appropriate, specific hypotheses being tested. examples

“The primary objective of this study was to investigate the cellular immune response to MSC injected into the striatum of allogeneic recipients (6-hydroxydopamine [6-OHDA]-hemilesioned rats, an animal model of Parkinson's disease [PD]), and the secondary objective was to determine the ability of these cells to prevent nigrostriatal dopamine depletion and associated motor deficits in these animals.” [1]

“In this exploratory study, we aimed to investigate whether calcium electroporation could initiate an anticancer immune response similar to electrochemotherapy. To this end, we treated immunocompetent balb/c mice with CT26 colon tumors with calcium electroporation, electrochemotherapy, or ultrasound-based delivery of calcium or bleomycin.” [2]

“While characterizing a rab-6.2-null C. elegans strain for another study, we observed that rab-6.2(ok2254) animals were fragile. We set out to analyze the fragile-skin phenotype in rab-6.2(ok2254) animals genetically…We observed several ruptured animals on our rab-6.2(ok2254) culture plates during normal maintenance, a phenotype very rarely observed in wild-type cultures…We hypothesized that RAB-6.2 is required for skin integrity.” [3]