Essential 10

2. Sample size Specify the exact number of experimental units allocated to each group, and the total number in each experiment. Also indicate the total number of animals used. examples

2a Specify the exact number of experimental units allocated to each group, and the total number in each experiment. Also indicate the total number of animals used.
2b Explain how the sample size was decided. Provide details of any a priori sample size calculation, if done.
Explanation
Examples

Example 1

Treatment and transplantation received by the animals for each group[1]

“Treatment and transplantation received by the animals for each group. The group were named after the treatment they received in treatment phase 1 and 2: S = saline, L = L-DOPA thus, SS group received saline in treatment phase 1 and 2, SL group received saline in treatment phase 1 and L-DOPA in treatment phase 2, LS received L-DOPA in Treatment phase 1 and saline in treatment phase 2 and LL received L-DOPA in treatment phase 1 and 2. *These groups originally numbered 12 animals but some developed tumors unrelated to the experiment, so had be removed from the study.” [1]

  1. Breger LS, Kienle K, Smith GA, Dunnett SB and Lane EL (2017). Influence of chronic L-DOPA treatment on immune response following allogeneic and xenogeneic graft in a rat model of Parkinson's disease. Brain Behav Immun. doi: 10.1016/j.bbi.2016.11.014