Essential 10

7. Statistical methods Describe any methods used to assess whether the data met the assumptions of the statistical approach, and what was done if the assumptions were not met. examples

7b Describe any methods used to assess whether the data met the assumptions of the statistical approach, and what was done if the assumptions were not met.
Examples

Example 1

“Model assumptions were checked using the Shapiro-Wilk normality test and Levene’s Test for homogeneity of variance and by visual inspection of residual and fitted value plots. Some of the response variables had to be transformed by applying the natural logarithm or the second or third root, but were back-transformed for visualization of significant effects.” [1] 

Example 2

“The effects of housing (treatment) and day of euthanasia on cortisol levels were assessed by using fixed-effects 2-way ANOVA. An initial exploratory analysis indicated that groups with higher average cortisol levels also had greater variation in this response variable. To make the variation more uniform, we used a logarithmic transform of each fish's cortisol per unit weight as the dependent variable in our analyses. This action made the assumptions of normality and homoscedasticity (standard deviations were equal) of our analyses reasonable.” [2]  

  1. Nemeth M, Millesi E, Wagner K-H and Wallner B (2015). Sex-specific effects of diets high in unsaturated fatty acids on spatial learning and memory in guinea pigs. PLOS ONE. doi: 10.1371/journal.pone.0140485
  2. Keck VA, Edgerton DS, Hajizadeh S, Swift LL, Dupont WD, Lawrence C and Boyd KL (2015). Effects of habitat complexity on pair-housed zebrafish. J Am Assoc Lab Anim Sci. https://www.ncbi.nlm.nih.gov/pubmed/26224437