Essential 10

9. Experimental procedures When and how often. examples

For each experimental group, including controls, describe the procedures in enough detail to allow others to replicate them, including:

9a What was done, how it was done, and what was used.
9b When and how often.
9c Where (including detail of any acclimatisation periods).
9d Why (provide rationale for procedures).
Explanation
Examples

Example 1

“Blood pressure, heart rate, oxygen saturation and amount of blood extracted were recorded every 5 minutes. Blood samples were drawn at baseline (pre injury), 0 minutes (immediately after injury), and after 30 and 60 minutes.” [1]

Example 2

“After a 5-h fast (7:30–12:30am), awake and freely moving mice were randomized and subjected to three consecutive clamps performed in the same mice as described above, with a 2 days recovery after each hyperinsulinemic/hypoglycemic (mHypo, n = 6) or hyperinsulinemic/euglycemic (mEugly, n = 4) clamps.” [2]

  1. Hagemo JS, Jørgensen JJ, Ostrowski SR, Holtan A, Gundersen Y, Johansson PI, Næss PA and Gaarder C (2013). Changes in fibrinogen availability and utilization in an animal model of traumatic coagulopathy. Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine. doi: 10.1186/1757-7241-21-56
  2. Emery M, Nanchen N, Preitner F, Ibberson M and Roduit R (2016). Biological Characterization of Gene Response to Insulin-Induced Hypoglycemia in Mouse Retina. PLOS ONE. doi: 10.1371/journal.pone.0150266