Essential 10

9. Experimental procedures Why (provide rationale for procedures). explanation

For each experimental group, including controls, describe the procedures in enough detail to allow others to replicate them, including:

9a What was done, how it was done, and what was used.
9b When and how often.
9c Where (including detail of any acclimatisation periods).
9d Why (provide rationale for procedures).
Explanation
Examples

There may be numerous approaches to investigate any given research problem, therefore it is important to explain why a particular procedure or technique was chosen. This is especially relevant when procedures are novel or specific to a research laboratory, or constrained by the animal model or experimental equipment (e.g. route of administration determined by animal size [1]).

 

References

  1. Turner PV, Brabb T, Pekow C and Vasbinder MA (2011). Administration of substances to laboratory animals: routes of administration and factors to consider. Journal of the American Association for Laboratory Animal Science : JAALAS. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3189662/