Essential 10

9. Experimental procedures Where (including detail of any acclimatisation periods). examples

For each experimental group, including controls, describe the procedures in enough detail to allow others to replicate them, including:

9a What was done, how it was done, and what was used.
9b When and how often.
9c Where (including detail of any acclimatisation periods).
9d Why (provide rationale for procedures).
Explanation
Examples

Example 1

“Fish were singly housed for 1 week before being habituated to the conditioning tank over 2 consecutive days. The conditioning tank consisted of an opaque tank measuring 20 cm (w) 15 cm (h) 30 cm (l) containing 2.5 l of aquarium water with distinct visual cues (spots or stripes) on walls at each end of the tank…During habituation, each individual fish was placed in the conditioning apparatus for 20 minutes with free access to both compartments and then returned to its home tank.” [1]

Brock AJ, Goody SMG, Mead AN, Sudwarts A, Parker MO and Brennan CH (2017). Assessing the Value of the Zebrafish Conditioned Place Preference Model for Predicting Human Abuse Potential. The Journal of pharmacology and experimental therapeutics. doi: 10.1124/jpet.117.242628