9. Experimental procedures Why (provide rationale for procedures). examples

“Because of the very small caliber of the murine tail veins, partial paravenous injection is common if 18F-FDG is administered by tail vein injection (intravenous). This could have significantly biased our comparison of the biodistribution of 18F-FDG under various conditions. Therefore, we used intraperitoneal injection of 18F-FDG for our experiments evaluating the influence of animal handling on 18F-FDG biodistribution.” [1]

“Since Xenopus oocytes have a higher potential for homologous recombination than fertilized embryos…we next tested whether the host transfer method could be used for efficient HDR-mediated knock-in.  We targeted the C-terminus of X. laevis Ctnnb1 (β-catenin), a key cytoskeletal protein and effector of the canonical Wnt pathway, because previous studies have shown that addition of epitope tags to the C-terminus do not affect the function of the resulting fusion protein (Fig…)…CRISPR components were injected into X. laevis oocytes followed by host transfer or into embryos.” [2]